A. 
The needle bars and tubes shall be cleaned by use of an ultrasonic cleaner prior to sterilization. Immersion time for all equipment shall be at least five minutes. Ultrasonic cleaning tank detergent shall be changed daily. The tank shall be scrubbed thoroughly with isopropyl alcohol between detergent changes.
B. 
Any establishment licensed after the date of this chapter shall have an autoclave on site for the sterilization of rods, needles, tubes, needle bars and other instruments used in the processes. Boiling is not a sterilizing option.
C. 
Instruments used in tattooing, such as needle bars, grips and tubes, shall be individually packaged and sterilized in an autoclave before use on each patron. Autoclaving shall be done under 15 pounds of pressure for 15 minutes at 126° C. or for 20 minutes at 121° C. Instrument sterilization bags shall have autoclave indicator tape and shall be dated. Autoclaves shall be maintained in accordance with manufacturer's recommendations, and a record of all maintenance shall be kept which includes the date and type of maintenance activity.
(1) 
The sterilization date shall be noted on each instrument pack. Sterile instruments shall be used within 30 days or shall be repackaged and resterilized.
(2) 
After completion of the procedure, all individual use items shall be disposed of and the area cleaned with a disinfectant solution.
D. 
If autoclaving is the method of sterilization, all tubes, grips and needle bars shall be left in the wrappers used during the autoclaving process until use.
E. 
Boiling is not an approved method of sterilization.
F. 
Needles, if used, shall be used only once and discarded.
G. 
Under no circumstances shall any instrument to be sterilized be coated with petrolatum, lubricating jelly, glycerin, oil or grease before being sterilized.
H. 
The tubes (holders for the stainless steel needle bars) shall also be sterilized together with other instruments used.
I. 
The motors or frames cannot be sterilized and they must be damp-wiped with seventy-percent alcohol or with freshly made one-and-five-tenths-percent solution of clean and soluble phenolic (carbolic acid) or with activated aqueous glutaraldehyde (e.g., Cidex).
The floor of the operating room of the tattoo establishment shall be of impervious material and shall be, at all times, maintained in a clean condition. The walls and ceiling of the operating room shall be a light color, shall be maintained in good repair without flaking or chipping and shall be of such material as to permit cleansing.
A. 
The tattoo establishment shall have proper facilities for the disposition of biomedical waste materials as now defined by state or federal regulations and as subsequently defined. A contract with an approved, licensed biomedical waste company is required and a copy of such contract shall be required at the time of licensing.
B. 
Sufficient toilet, urinal and hand-washing facilities shall be accessible to customers, operators and artists within the tattoo establishment or the building in which said tattoo establishment is located.